As Bluetooth and WiFi both use the 2.4GHz band, is it possible that they can interfere with one another? John-Paul Little has a old post on Can iBeacon deployments cause WI-FI interference. The conclusion is no, it won’t, as there’s negligible overlap between the WiFi and Beacon frequencies:
The 2.4GHz band is split into smaller frequencies, called channels, such that more than one technology can share the same band.
Also, at the practical level, we have used WiFi and (many) Beacons together on many projects and we have never experienced a problem. Although still rare, it’s more likely that multiple Bluetooth devices can interfere.